PSMD9 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PSMD9 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H2G1

Cat no : 67338-1-PBS

Synonyms

p27, PSMD9, Rpn4

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Validation Data Gallery

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  WB analysis using 67338-1-Ig (same clone as 67338-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67338-1-Ig (PSMD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 67338-1-Ig (same clone as 67338-1-PBS)

  WB result of PSMD9 antibody (67338-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD9 transfected A549 cells. This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67338-1-Ig (same clone as 67338-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67338-1-Ig (PSMD9 antibody) at dilution of 1:2800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67338-1-Ig (same clone as 67338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67338-1-Ig (PSMD9 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67338-1-Ig (same clone as 67338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67338-1-Ig (PSMD9 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67338-1-Ig (same clone as 67338-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMD9 antibody (67338-1-Ig, Clone: 1H2G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67338-1-Ig (same clone as 67338-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMD9 antibody (67338-1-Ig, Clone: 1H2G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67338-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67338-1-PBS targets PSMD9 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSMD9 fusion protein Ag25654
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC004213
• Gene Symbol: PSMD9
• Gene ID (NCBI): 5715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PSMD9 is a ubiquitous protein of eukaryotic cells and is a chaperon of the 26S proteasome complex, which degrades ubiquitinated proteins in eukaryotic cells and contributes to the degradation of intracellular proteins into antigenic peptides for antigen presentation by MHC class I cells. The 26S mammalian base sub-complex involves three distinct modules which have ATPase subunits distinctly associated to three chaperones, one of which is PSMD9 regulating the modules assembly. The PSMD9 ubiquitous regulatory role within the proteasome implies its potential pleiotropic effects within different physio-pathological systems. PSMD9 is known to form a stable subcomplex with PSMC3 and PSMC6, two of the AAA-ATPases, assisting in the assembly of the 20S and 19S particles to form the holo complex.