PSMD14/POH1 Recombinant antibody

PSMD14/POH1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242160E7

Cat no : 84767-3-RR

Synonyms

PSMD14, POH1, PAD1, EC:3.4.19.-, 26S proteasome-associated PAD1 homolog 1

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Validation Data Gallery

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  WB analysis using 84767-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 84767-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 84767-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A375 using 84767-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using PSMD14/POH1 antibody (84767-3-RR, Clone: 242160E7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84767-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84767-3-RR against Human PSMD14/POH1 were performed. The affinity constant is 3.60 nM.

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Tested Applications

• Positive WB detected in: A431 cells, K-562 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, mouse heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84767-3-RR targets PSMD14/POH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PSMD14/POH1 fusion protein Ag2694
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC009524
• Gene Symbol: PSMD14
• Gene ID (NCBI): 10213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PSMD14 (POH1, also known as Rpn11/MPR1/S13/CepP1) protein is a metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. PSMD14 is highly expressed in the heart and skeletal muscle. In carcinoma cell lines. down-regulation of PSMD14 by siRNA transfection had a considerable impact on cell viability causing cell arrest in the G0-G1 phase, ultimately leading to senescence.