PSMD14/POH1 Recombinant antibody, PBS Only (Capture/Detector)

PSMD14/POH1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242160E7

Cat no : 84767-3-PBS

Synonyms

PSMD14, POH1, PAD1, EC:3.4.19.-, 26S proteasome-associated PAD1 homolog 1

---

Validation Data Gallery

• 

  WB analysis using 84767-3-RR (same clone as 84767-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84767-3-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01566-3

  Sandwich ELISA standard curve of MP01566-3, Human PSMD14/POH1 Recombinant Matched Antibody Pair - PBS only. 84767-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2694. 84767-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Sample test of MP01566-3

  The mean PSMD14/POH1 concentration was determined to be 12.08 ng/mL in A431 cell extract based on a 1.30 mg/mL extract load, 24.32 ng/mL in K-562 cell extract based on a 2.40 mg/mL extract load and 10.64 ng/mL in HT-1080 cell extract based on a 1.40 mg/mL extract load.

• 

  IHC staining of mouse heart using 84767-3-RR (same clone as 84767-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84767-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 84767-3-RR (same clone as 84767-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84767-3-PBS in a different storage buffer formulation.

• 

  IF Staining of A375 using 84767-3-RR (same clone as 84767-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using PSMD14/POH1 antibody (84767-3-RR, Clone: 242160E7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84767-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01566-1

  Cytometric bead array standard curve of MP01566-1, PSMD14/POH1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84767-3-PBS. Detection antibody: 84767-2-PBS. Standard: Ag2694. Range: 0.781-50 ng/mL.

• 

  Cytometric bead array standard curve of MP01566-2

  Cytometric bead array standard curve of MP01566-2, PSMD14/POH1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84767-3-PBS. Detection antibody: 84767-1-PBS. Standard: Ag2694. Range: 0.781-50 ng/mL.

• 

  Affinity and Kinetic Characterization of 84767-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84767-3-RR against Human PSMD14/POH1 were performed. The affinity constant is 3.60 nM.

---

Product Information

84767-3-PBS targets PSMD14/POH1 as part of a matched antibody pair:

MP01566-1: 84767-3-PBS capture and 84767-2-PBS detection (validated in Cytometric bead array)

MP01566-2: 84767-3-PBS capture and 84767-1-PBS detection (validated in Cytometric bead array)

MP01566-3: 84767-1-PBS capture and 84767-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PSMD14/POH1 fusion protein Ag2694
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC009524
• Gene Symbol: PSMD14
• Gene ID (NCBI): 10213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The PSMD14 (POH1, also known as Rpn11/MPR1/S13/CepP1) protein is a metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. PSMD14 is highly expressed in the heart and skeletal muscle. In carcinoma cell lines. down-regulation of PSMD14 by siRNA transfection had a considerable impact on cell viability causing cell arrest in the G0-G1 phase, ultimately leading to senescence.