CoraLite® Plus 488-conjugated PSMB5 Monoclonal antibody

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  IF Staining of HeLa using CL488-67959

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PSMB5 antibody (CL488-67959, Clone: 2E9H1 ) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HeLa using CL488-67959

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated PSMB5 Monoclonal antibody (CL488-67959, Clone:2E9H1)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

PSMB5 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-67959

Clone No.2E9H1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

X, Proteasome subunit beta type-5, MB1, LMPX, EC:3.4.25.1

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67959 targets PSMB5 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5828

  Product name: Recombinant human PSMB5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-263 aa of BC057840

  Sequence: MALASVLERPLPVNQRGFFGLGGRADLLDLGPGSLSDGLSLAAPGWGVPEEPGIEMLHGTTTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGSTP

• Full Name: proteasome (prosome, macropain) subunit, beta type, 5
• Calculated Molecular Weight: 263 aa, 28 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC057840
• Gene Symbol: PSMB5
• Gene ID (NCBI): 5693
• RRID: AB_3084387
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P28074
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.