PSMA2 Monoclonal antibody

PSMA2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H4A10

Cat no : 68240-1-Ig

Synonyms

HC3, Macropain subunit C3, MU, PMSA2, Proteasome component C3, PSC2, PSC3, PSMA2

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Validation Data Gallery

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  WB analysis using 68240-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68240-1-Ig (PSMA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 68240-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68240-1-Ig (PSMA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 68240-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68240-1-Ig (PSMA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U2OS cells, A431 cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, pig heart tissue, rat heart tissue, mouse heart tissue
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68240-1-Ig targets PSMA2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

• Tested Reactivity: Human, mouse, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSMA2 fusion protein Ag5837
• Full Name: proteasome (prosome, macropain) subunit, alpha type, 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC047697
• Gene Symbol: PSMA2
• Gene ID (NCBI): 5683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMA2 (Proteasome subunit alpha type-2) is the essential subunit that contributes to the complete assembly of 20S proteasome complex, involved in the proteolytic degradation of most intracellular proteins. It is required in spermatogenesis and generation of a subset of MHC class I-presented antigenic peptides(PMID: 34711951, PMID: 27342858). Dysregulation of PSMA2 degradation leads to tumorigenesis including cervical cancer (PMID:33684886).