CoraLite® Plus 488-conjugated PSMA/GCPII Monoclonal antibody

PSMA/GCPII Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G4E12

Cat no : CL488-66678

Synonyms

FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP

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Validation Data Gallery

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  IF Staining of human prostate cancer using CL488-66678

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 PSMA/GCPII antibody (CL488-66678, Clone: 3G4E12 ) at dilution of 1:200.

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  IF Staining of human prostate cancer using CL488-66678

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 PSMA/GCPII antibody (CL488-66678, Clone: 3G4E12 ) at dilution of 1:200.

• 

  IF Staining of PC-3 using CL488-66678

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 PSMA/GCPII antibody (CL488-66678, Clone: 3G4E12 ) at dilution of 1:100.

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Tested Applications

• Positive IF-P detected in: human prostate cancer tissue, PC-3 cells
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66678 targets PSMA/GCPII in IF/ICC, IF-P applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSMA/GCPII fusion protein Ag16594
• Full Name: folate hydrolase (prostate-specific membrane antigen) 1
• Calculated Molecular Weight: 719 aa, 81 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC025672
• Gene Symbol: PSMA
• Gene ID (NCBI): 2346
• RRID: AB_2919356
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.