CoraLite® Plus 488-conjugated PRX5 Monoclonal antibody

PRX5 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B8C5

Cat no : CL488-67599

Synonyms

Peroxiredoxin 5, PRDX5, B166, AOPP, AOEB166

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Validation Data Gallery

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  IF Staining of HeLa using CL488-67599

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PRX5 antibody (CL488-67599, Clone: 1B8C5 ) at dilution of 1:600.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67599 targets PRX5 in IF/ICC applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRX5 fusion protein Ag12262
• Full Name: peroxiredoxin 5
• Calculated Molecular Weight: 214 aa, 22 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC110983
• Gene Symbol: PRX5
• Gene ID (NCBI): 25824
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Peroxiredoxin 5 (Prx5) is a member of a family consisting of six antioxidant enzymes. Prx5 is ubiquitously expressed in various tissues including mitochondria and peroxisomes, implying that Prx5 functions as a regulator of the cellular oxidation state. Prx5 plays a critical role in protecting cells from oxidative stress by inhibiting the accumulation of reactive oxygen species and cell death. Prx5 has an intensive ROS scavenging activity because it is located in the cytosol and mitochondria. Prx5 expression was upregulated during adipogenesis and Prx5 overexpression suppressed adipogenesis by regulating cytosolic and mitochondrial ROS generation. (PMID: 29777756, PMID: 31505325, PMID: 22020876)