CoraLite® Plus 488-conjugated PRDX3 Monoclonal antibody

PRDX3 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F7F7

Cat no : CL488-66810

Synonyms

MER5, EC:1.11.1.24, AOP-1, AOP1, Antioxidant protein 1

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Validation Data Gallery

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  FC experiment of MCF-7 using CL488-66810

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PRX3 (CL488-66810, Clone:1F7F7) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66810 targets PRDX3 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRDX3 fusion protein Ag1062
• Full Name: peroxiredoxin 3
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC007062
• Gene Symbol: PRDX3
• Gene ID (NCBI): 10935
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C