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  • Featured Product
  • KD/KO Validated

PRUNE2 Polyclonal antibody, PBS Only

PRUNE2 Polyclonal Antibody for IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11458-1-PBS

Synonyms

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRUNE2 Polyclonal antibody (11458-1-AP)

    IHC staining of human liver cancer using 11458-1-AP (same clone as 11458-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11458-1-AP (PRUNE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11458-1-PBS in a different storage buffer formulation.

Product Information

11458-1-PBS targets PRUNE2 in IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRUNE2 fusion protein Ag2025
Full Name prune homolog 2 (Drosophila)
Calculated Molecular Weight 341 kDa
Observed Molecular Weight 140~150 kDa
GenBank Accession NumberBC019095
Gene Symbol PRUNE2
Gene ID (NCBI) 158471
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PRUNE2 gene, also named BMCC1, encodes protein prune homolog 2. PRUNE2 gene are overlapping with PCA3, one of the most prostate cancer specific markers. PRUNE2 may play an important role in regulating differentiation, survival and aggressiveness of the tumor cells. Besides its high level of expression seen in the nervous system (brain, cerebellum and spinal cord), it is also expressed at high levels in noneuroblastoma, rhabdomyosarcoma, melanoma and some osteosarcoma cell lines, whereas at only low levels in cancer cell lines of liver, breast, thyroid and colon. The Calculated molecular weight of this protein is 340 kDa. Our antibody specifically recognises the 140-150 kDa band which is an isoform or cleaved fragment of PRUNE2 (PMID:21234814).