PRUNE2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRUNE2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11458-1-AP

Synonyms

A214N16.3, BMCC1, BNIPXL, C9orf65, KIAA0367, Protein prune homolog 2, prune homolog 2 (Drosophila), PRUNE2, RP11 58J3.2

---

Validation Data Gallery

• 

  IHC staining of human liver cancer using 11458-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11458-1-AP (PRUNE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11458-1-AP targets PRUNE2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRUNE2 fusion protein Ag2025
• Full Name: prune homolog 2 (Drosophila)
• Calculated Molecular Weight: 341 kDa
• Observed Molecular Weight: 140~150 kDa
• GenBank Accession Number: BC019095
• Gene Symbol: PRUNE2
• Gene ID (NCBI): 158471
• RRID: AB_2172439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRUNE2 gene, also named BMCC1, encodes protein prune homolog 2. PRUNE2 gene are overlapping with PCA3, one of the most prostate cancer specific markers. PRUNE2 may play an important role in regulating differentiation, survival and aggressiveness of the tumor cells. Besides its high level of expression seen in the nervous system (brain, cerebellum and spinal cord), it is also expressed at high levels in noneuroblastoma, rhabdomyosarcoma, melanoma and some osteosarcoma cell lines, whereas at only low levels in cancer cell lines of liver, breast, thyroid and colon. The Calculated molecular weight of this protein is 340 kDa. Our antibody specifically recognises the 140-150 kDa band which is an isoform or cleaved fragment of PRUNE2 (PMID:21234814).

Publications

Species	Application	Title
human	WB, IF, IP	Proc Natl Acad Sci U S A; PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3.; Authors - Ahmad Salameh ; KD Validated
human	WB, IHC	Cell Death Dis; BMCC1, which is an interacting partner of BCL2, attenuates AKT activity, accompanied by apoptosis.; Authors - Y Tatsumi
human	WB,IHC	Exp Ther Med; PRUNE2 inhibits progression of colorectal cancer in vitro and in vivo.; Authors - Ting Li ; KD Validated
human,mouse	IHC,WB	Technol Cancer Res Treat; FOXF2 Regulates PRUNE2 Transcription in the Pathogenesis of Colorectal Cancer; Authors - Ting Li