PRMT6 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PRMT6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15395-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 15395-1-AP (same clone as 15395-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15395-1-AP (PRMT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 15395-1-AP (same clone as 15395-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15395-1-AP (PRMT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 15395-1-AP (same clone as 15395-1-PBS)

  IP result of anti-PRMT6 (IP:15395-1-AP, 4ug; Detection:15395-1-AP 1:1500) with HEK-293 cells lysate 2400ug. This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 15395-1-AP (same clone as 15395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 15395-1-AP (same clone as 15395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15395-1-AP (same clone as 15395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15395-1-AP (same clone as 15395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 15395-1-AP (same clone as 15395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 15395-1-AP (same clone as 15395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 15395-1-AP (same clone as 15395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 15395-1-AP (same clone as 15395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 15395-1-AP (same clone as 15395-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PRMT6 antibody (15395-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 15395-1-AP (same clone as 15395-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug PRMT6 Polyclonal antibody (15395-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

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Product Information

15395-1-PBS targets PRMT6 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRMT6 fusion protein Ag7592
• Full Name: protein arginine methyltransferase 6
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC002729
• Gene Symbol: PRMT6
• Gene ID (NCBI): 55170
• RRID: AB_2237723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRMT6 belongs to the protein arginine methyltransferase (PRMT) family that catalyzes the mono- and dimethylation of arginine residues in a variety of proteins. PRMT6 is a nuclear protein and is predominantly expressed in testis and kidney. Western blot analysis using this antibody detected a major band around 42 kDa in HeLa cells.