PRMT3 Recombinant antibody, PBS Only

PRMT3 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241092H2

Cat no : 83969-6-PBS

Synonyms

Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319

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Validation Data Gallery

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  WB analysis using 83969-6-RR (same clone as 83969-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83969-6-RR (PRMT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83969-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 83969-6-RR (same clone as 83969-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83969-6-RR (PRMT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83969-6-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83969-6-RR (same clone as 83969-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRMT3 antibody (83969-6-RR, Clone: 241092H2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83969-6-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83969-6-RR (same clone as 83969-6-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PRMT3 Recombinant antibody (83969-6-RR, Clone:241092H2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83969-6-PBS in a different storage buffer formulation.

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Product Information

The immunogen of 83969-6-PBS is PRMT3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PRMT3 fusion protein Ag13466
• Full Name: protein arginine methyltransferase 3
• Calculated Molecular Weight: 531 aa, 60 kDa
• Observed Molecular Weight: 60-68 kDa
• GenBank Accession Number: BC037544
• Gene Symbol: PRMT3
• Gene ID (NCBI): 10196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRMT3(protein arginine methyltransferase 3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins.