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PRMT3 Recombinant antibody

PRMT3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241092H2

Cat no : 83969-6-RR

Synonyms

Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRMT3 Recombinant antibody (83969-6-RR)

    WB analysis using 83969-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83969-6-RR (PRMT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PRMT3 Recombinant antibody (83969-6-RR)

    IHC staining of mouse liver using 83969-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83969-6-RR (PRMT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRMT3 Recombinant antibody (83969-6-RR)

    IF Staining of HepG2 using 83969-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRMT3 antibody (83969-6-RR, Clone: 241092H2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of MCF-7 cells using PRMT3 Recombinant antibody (83969-6-RR)

    FC experiment of MCF-7 using 83969-6-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PRMT3 Recombinant antibody (83969-6-RR, Clone:241092H2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells, SW480 cells, HEK-293 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83969-6-RR is PRMT3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PRMT3 fusion protein Ag13466
Full Name protein arginine methyltransferase 3
Calculated Molecular Weight 531 aa, 60 kDa
Observed Molecular Weight60-68 kDa
GenBank Accession NumberBC037544
Gene Symbol PRMT3
Gene ID (NCBI) 10196
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRMT3(protein arginine methyltransferase 3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins.

Protocols

Product Specific Protocols
WB protocol for PRMT3 antibody 83969-6-RRDownload protocol
IHC protocol for PRMT3 antibody 83969-6-RRDownload protocol
IF protocol for PRMT3 antibody 83969-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols