PRKCB Monoclonal antibody, PBS Only

PRKCB Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit, rat, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4B7A1

Cat no : 68620-1-PBS

Synonyms

PKC B, PKC beta, PKCB, PRKCB, PRKCB1, PRKCB2, Protein kinase C beta type, protein kinase C, beta

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Validation Data Gallery

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  WB analysis using 68620-1-Ig (same clone as 68620-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68620-1-Ig (PRKCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68620-1-PBS in a different storage buffer formulation.

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Product Information

68620-1-PBS targets PRKCB in WB, Indirect ELISA applications and shows reactivity with Human, pig, rabbit, rat, mouse samples.

• Tested Reactivity: Human, pig, rabbit, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRKCB fusion protein Ag33693
• Full Name: protein kinase C, beta
• Calculated Molecular Weight: 673 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC036472
• Gene Symbol: PRKCB
• Gene ID (NCBI): 5579
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.