PRKCB Monoclonal antibody
PRKCB Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, pig, rabbit, rat, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
4B7A1
Cat no : 68620-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human brain tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IHC | See 1 publications below |
Product Information
68620-1-Ig targets PRKCB in WB, IHC, ELISA applications and shows reactivity with Human, pig, rabbit, rat, mouse samples.
Tested Reactivity | Human, pig, rabbit, rat, mouse |
Cited Reactivity | rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PRKCB fusion protein Ag33693 |
Full Name | protein kinase C, beta |
Calculated Molecular Weight | 673 aa, 77 kDa |
Observed Molecular Weight | 77 kDa |
GenBank Accession Number | BC036472 |
Gene Symbol | PRKCB |
Gene ID (NCBI) | 5579 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.
Protocols
Product Specific Protocols | |
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WB protocol for PRKCB antibody 68620-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Redox Biol Empagliflozin prevents oxidative stress in human coronary artery endothelial cells via the NHE/PKC/NOX axis | ||
J Ethnopharmacol Omics-based pharmacological evaluation reveals Yuanhu Zhitong oral liquid ameliorates arthritis by regulating PKC/ERK/NF-κB signaling pathway |