PRKCB Monoclonal antibody

PRKCB Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4B7A1

Cat no : 68620-1-Ig

Synonyms

PKC B, PKC beta, PKCB, PRKCB, PRKCB1, PRKCB2, Protein kinase C beta type, protein kinase C, beta



Tested Applications

Positive WB detected inhuman brain tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68620-1-Ig targets PRKCB in WB, IHC, ELISA applications and shows reactivity with Human, pig, rabbit, rat, mouse samples.

Tested Reactivity Human, pig, rabbit, rat, mouse
Cited Reactivityrat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PRKCB fusion protein Ag33693
Full Name protein kinase C, beta
Calculated Molecular Weight 673 aa, 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC036472
Gene Symbol PRKCB
Gene ID (NCBI) 5579
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.

Protocols

Product Specific Protocols
WB protocol for PRKCB antibody 68620-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Redox Biol

Empagliflozin prevents oxidative stress in human coronary artery endothelial cells via the NHE/PKC/NOX axis

Authors - Xiaoling Li
ratWB,IHC

J Ethnopharmacol

Omics-based pharmacological evaluation reveals Yuanhu Zhitong oral liquid ameliorates arthritis by regulating PKC/ERK/NF-κB signaling pathway

Authors - Kai Zhang