PRDX4 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PRDX4 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5B6F1

Cat no : 60286-1-PBS

Synonyms

Antioxidant enzyme AOE372, AOE37 2, peroxiredoxin 4, Peroxiredoxin IV, PRDX4, PRX 4, Prx IV, Thioredoxin peroxidase AO372

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Validation Data Gallery

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  WB analysis of HepG2 using 60286-1-Ig (same clone as 60286-1-PBS)

  WB result of PRDX4 antibody (60286-1-Ig, 1:5000) with si-Control and si-PRDX4 transfected HepG2 cells. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  WB analysis using 60286-1-Ig (same clone as 60286-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60286-1-Ig (same clone as 60286-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 60286-1-Ig (same clone as 60286-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 60286-1-Ig (same clone as 60286-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 60286-1-Ig (same clone as 60286-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 60286-1-Ig (same clone as 60286-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60286-1-Ig (same clone as 60286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(unde10x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60286-1-Ig (same clone as 60286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60286-1-Ig (same clone as 60286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60286-1-Ig (same clone as 60286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60286-1-Ig (same clone as 60286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60286-1-Ig (same clone as 60286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60286-1-Ig (same clone as 60286-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 60286-1-Ig (PRDX4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60286-1-PBS targets PRDX4 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRDX4 fusion protein Ag13401
• Full Name: peroxiredoxin 4
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC007107
• Gene Symbol: PRDX4
• Gene ID (NCBI): 10549
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503).