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  • Featured Product
  • KD/KO Validated

PRDX4 Monoclonal antibody

PRDX4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5B6F1

Cat no : 60286-1-Ig

Synonyms

Peroxiredoxin IV, peroxiredoxin 4, EC:1.11.1.24, AOE37-2, AOE37 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PRDX4 Monoclonal antibody (60286-1-Ig)

    WB analysis of HepG2 using 60286-1-Ig

    WB result of PRDX4 antibody (60286-1-Ig, 1:5000) with si-Control and si-PRDX4 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using PRDX4 Monoclonal antibody (60286-1-Ig)

    WB analysis using 60286-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PRDX4 Monoclonal antibody (60286-1-Ig)

    WB analysis of NIH/3T3 using 60286-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using PRDX4 Monoclonal antibody (60286-1-Ig)

    WB analysis of PC-12 using 60286-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PRDX4 Monoclonal antibody (60286-1-Ig)

    WB analysis of K-562 using 60286-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PRDX4 Monoclonal antibody (60286-1-Ig)

    WB analysis of HeLa using 60286-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PRDX4 Monoclonal antibody (60286-1-Ig)

    WB analysis of HepG2 using 60286-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using PRDX4 Monoclonal antibody (60286-1-Ig)

    IHC staining of human colon using 60286-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(unde10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using PRDX4 Monoclonal antibody (60286-1-Ig)

    IHC staining of human colon using 60286-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PRDX4 Monoclonal antibody (60286-1-Ig)

    IHC staining of human prostate cancer using 60286-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PRDX4 Monoclonal antibody (60286-1-Ig)

    IHC staining of human prostate cancer using 60286-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PRDX4 Monoclonal antibody (60286-1-Ig)

    IHC staining of human pancreas cancer using 60286-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PRDX4 Monoclonal antibody (60286-1-Ig)

    IHC staining of human pancreas cancer using 60286-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRDX4 Monoclonal antibody (60286-1-Ig)

    IF Staining of HepG2 using 60286-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PRDX4 antibody (60286-1-Ig, Clone: 5B6F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HepG2 cells, K-562 cells, PC-12 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected inhuman colon tissue, human pancreas cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60286-1-Ig targets PRDX4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PRDX4 fusion protein Ag13401
Full Name peroxiredoxin 4
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC007107
Gene Symbol PRDX4
Gene ID (NCBI) 10549
RRIDAB_2881403
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503).

Protocols

Product Specific Protocols
WB protocol for PRDX4 antibody 60286-1-IgDownload protocol
IHC protocol for PRDX4 antibody 60286-1-IgDownload protocol
IF protocol for PRDX4 antibody 60286-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma

Authors - Alexander Vdovin
  • KD Validated
View Article
mouseWB

Biomolecules

Exosome-Related FTCD Facilitates M1 Macrophage Polarization and Impacts the Prognosis of Hepatocellular Carcinoma

Authors - Youyi Liu
View Article
mouseIF

Biochem Biophys Res Commun

Peroxiredoxin 4 secreted by cumulus cells ameliorates the maturation of oocytes in vitro

Authors - Wangjuan Dai
View Article