PRC1 Recombinant antibody, PBS Only (Capture)

PRC1 Uni-rAb^TM Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241216H2

Cat no : 84041-2-PBS

Synonyms

ASE1, 241216H2

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Validation Data Gallery

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  IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00970-1

  Cytometric bead array standard curve of MP00970-1, PRC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84041-2-PBS. Detection antibody: 84041-1-PBS. Standard: Ag8028. Range: 0.625-80 ng/mL.

• 

  Cytometric bead array standard curve of MP00970-2

  Cytometric bead array standard curve of MP00970-2, PRC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84041-2-PBS. Detection antibody: 84041-3-PBS. Standard:null. Range: null.

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  Cytometric bead array standard curve of MP00970-2

  Cytometric bead array standard curve of MP00970-2, PRC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84041-2-PBS. Detection antibody: 84041-3-PBS. Standard: Ag8028. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84041-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84041-2-RR against Human PRC1 were performed. The affinity constant is below 1 pM.

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Product Information

84041-2-PBS targets PRC1 as part of a matched antibody pair:

MP00970-1: 84041-2-PBS capture and 84041-1-PBS detection (validated in Cytometric bead array)

MP00970-2: 84041-2-PBS capture and 84041-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PRC1 fusion protein Ag8028
• Full Name: protein regulator of cytokinesis 1
• Calculated Molecular Weight: 72 kDa
• GenBank Accession Number: BC003138
• Gene Symbol: PRC1
• Gene ID (NCBI): 9055
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively.