PRAS40 Polyclonal antibody

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  WB analysis using 32456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32456-1-AP (PRAS40 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 32456-1-AP

  IP result of anti-PRAS40 (IP:32456-1-AP, 4ug; Detection:32456-1-AP 1:2000) with HeLa cells lysate 1480 ug.

PRAS40 Polyclonal Antibody for WB, IP, ELISA

Cat No. 32456-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

AKT1S1, 40 kDa proline rich AKT substrate, 40 kDa proline-rich AKT substrate, Proline rich AKT1 substrate 1, Proline-rich AKT1 substrate 1

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Tested Applications

• Positive WB detected in: A549 cells, Calu-1 cells, HeLa cells, HepG2 cells, L02 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32456-1-AP targets PRAS40 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRAS40 fusion protein Ag35446
• Full Name: AKT1 substrate 1 (proline-rich)
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC051844
• Gene Symbol: PRAS40
• Gene ID (NCBI): 84335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96B36
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRAS40 (40 kDa proline-rich AKT substrate) is also named as AKT1S1 (Proline-rich AKT1 substrate 1). PRAS40 is a substrate of Akt and a component of the mammalian target of rapamycin complex 1 (mTORC1) (PMID: 28978182). Locating at the crossroad of the PI3K/Akt pathway and the mTOR pathway, PRAS40 is phosphorylated by growth factors or other stimuli, and regulates the activation of these signaling pathways in turn (PMID: 28978182). PRAS40 is expressed in a variety of tissues in vivo and has multiple phosphorylation sites, which its activity is closely related to phosphorylation. Studies have shown that PRAS40 is involved in regulating cell growth, cell apoptosis, oxidative stress, autophagy and angiogenesis, as well as various of signalling pathways such as mammalian target of mammalian target rapamycin (mTOR), protein kinase B (PKB/Akt), nuclear factor kappa-B(NF-κB) proto-oncogene serine/threonine-protein kinase PIM-1(PIM1) and pyruvate kinase M2 (PKM2) (PMID: 33504218).