PRAME Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PRAME Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E9G9

Cat no : 68097-1-PBS

Synonyms

MAPE, OIP 4, OIP4, Opa interacting protein 4, PRAME

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Validation Data Gallery

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  WB analysis using 68097-1-Ig (same clone as 68097-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68097-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68097-1-Ig (same clone as 68097-1-PBS)

  WB result of PRAME antibody (68097-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRAME transfected HeLa cells. This data was developed using the same antibody clone with 68097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 68097-1-Ig (same clone as 68097-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 68097-1-Ig (same clone as 68097-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 68097-1-Ig (same clone as 68097-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68097-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 68097-1-Ig (same clone as 68097-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68097-1-PBS in a different storage buffer formulation.

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  IF Staining of HaCaT using 68097-1-Ig (same clone as 68097-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using PRAME antibody (68097-1-Ig, Clone: 1E9G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68097-1-PBS in a different storage buffer formulation.

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Product Information

68097-1-PBS targets PRAME in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRAME fusion protein Ag1906
• Full Name: preferentially expressed antigen in melanoma
• Calculated Molecular Weight: 509 aa, 58 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC014074
• Gene Symbol: PRAME
• Gene ID (NCBI): 23532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The PRAME (preferentially expressed antigen of melanoma) gene was previously shown to be overexpressed in ovarian/primary peritoneal serous carcinoma compared with malignant mesothelioma using gene expression arrays. It is considered a melanocyte differentiation antigen which is overexpressed in both solid and hematologic tumors. In normal tissue, a very low level of PRAME expression is found in normal testis, adrenals, ovary and endometrium. A high level of PRAME expression has been reported for several solid tumors, including ovarian cancer, breast cancer, lung cancer and melanomas, medulloblastoma, sarcomas, head and neck cancers, neuroblastoma, renal cancer, and Wilms'tumor. As a nuclear transcriptional repressor protein, PRAME binds to retinoic acid receptor a, thereby inhibiting retinoic acid induced differentiation, growth arrest, and apoptosis.