PPP3CA Monoclonal antibody, PBS Only

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  WB analysis using 68163-1-Ig (same clone as 68163-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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  WB analysis using 68163-1-Ig (same clone as 68163-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 68163-1-Ig (same clone as 68163-1-PBS)

  IP result of anti-PPP3CA (IP:68163-1-Ig, 5ug; Detection:68163-1-Ig 1:4000) with rat brain tissue lysate 1120 ug. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 68163-1-Ig (same clone as 68163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 68163-1-Ig (same clone as 68163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 68163-1-Ig (same clone as 68163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 68163-1-Ig (same clone as 68163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skeletal muscle using 68163-1-Ig (same clone as 68163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skeletal muscle using 68163-1-Ig (same clone as 68163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

PPP3CA Monoclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 68163-1-PBS

Clone No.2A5C1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

CALNA, CALNA1, CALN, Calmodulin-dependent calcineurin A subunit alpha isoform, Calcineurin A alpha

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Product Information

68163-1-PBS targets PPP3CA in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPP3CA fusion protein Ag32642
• Full Name: protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC025714
• Gene Symbol: PPP3CA
• Gene ID (NCBI): 5530
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q08209
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.