PPP3CA Monoclonal antibody

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  WB analysis using 68163-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis using 68163-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 68163-1-Ig

  IP result of anti-PPP3CA (IP:68163-1-Ig, 5ug; Detection:68163-1-Ig 1:4000) with rat brain tissue lysate 1120 ug.

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  IHC staining of human thyroid cancer using 68163-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 68163-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 68163-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 68163-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skeletal muscle using 68163-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skeletal muscle using 68163-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PPP3CA Monoclonal Antibody for WB, IHC, IP, ELISA

Cat No. 68163-1-Ig

Clone No.2A5C1

Reviews (1)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IP, ELISA

Synonyms

CALNA, CALNA1, CALN, Calmodulin-dependent calcineurin A subunit alpha isoform, Calcineurin A alpha

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, rabbit skeletal muscle tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, rat skeletal muscle
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: human thyroid cancer tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68163-1-Ig targets PPP3CA in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPP3CA fusion protein Ag32642
• Full Name: protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC025714
• Gene Symbol: PPP3CA
• Gene ID (NCBI): 5530
• RRID: AB_2923682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q08209
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nora (Verified Customer) (02-05-2025)

Worked and showed all the protein isoforms

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: iPSC-CM