PPP1R13B Polyclonal antibody, PBS Only

PPP1R13B Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29804-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 29804-1-AP (same clone as 29804-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29804-1-AP (PPP1R13B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29804-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 29804-1-AP (same clone as 29804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29804-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 29804-1-AP (same clone as 29804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29804-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 29804-1-AP (same clone as 29804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29804-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 29804-1-AP (same clone as 29804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29804-1-PBS in a different storage buffer formulation.

---

Product Information

29804-1-PBS targets PPP1R13B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP1R13B fusion protein Ag30788
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 13B
• Calculated Molecular Weight: 1090 aa, 120 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC136527
• Gene Symbol: PPP1R13B
• Gene ID (NCBI): 23368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PPP1R13B(Protein phosphatase 1 regulatory subunit 13B), also named ASPP1 and KIAA0771, is a member of the apoptosis-stimulating proteins of the p53 family (ASPPs)(PMID: 30908929). PPP1R13B contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. PPP1R13 promotes DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor.