PPP1R13B Polyclonal antibody

PPP1R13B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29804-1-AP

Synonyms

ASPP1, KIAA0771, p53BP2 like, p85, PPP1R13B

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Validation Data Gallery

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  WB analysis using 29804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29804-1-AP (PPP1R13B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 29804-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 29804-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 29804-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 29804-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells
• Positive IHC detected in: human lung cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29804-1-AP targets PPP1R13B in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP1R13B fusion protein Ag30788
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 13B
• Calculated Molecular Weight: 1090 aa, 120 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC136527
• Gene Symbol: PPP1R13B
• Gene ID (NCBI): 23368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1R13B(Protein phosphatase 1 regulatory subunit 13B), also named ASPP1 and KIAA0771, is a member of the apoptosis-stimulating proteins of the p53 family (ASPPs)(PMID: 30908929). PPP1R13B contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. PPP1R13 promotes DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor.

Publications

Species	Application	Title
sheep	WB	Int J Biol Macromol; Estrogen-mediated oar-miR-485-5p targets PPP1R13B to regulate myoblast proliferation in sheep; Authors - Siqi Liu