PPID Recombinant antibody

PPID Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241361E4

Cat no : 84115-6-RR

Synonyms

Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase

---

Validation Data Gallery

• 

  WB analysis using 84115-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84115-6-RR (PPID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 84115-6-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PPID Recombinant antibody (84115-6-RR, Clone:241361E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 84115-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84115-6-RR against Human PPID were performed. The affinity constant is 0.497 nM.

---

Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84115-6-RR is PPID Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PPID fusion protein Ag3409
• Full Name: peptidylprolyl isomerase D
• Calculated Molecular Weight: 370 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC030707
• Gene Symbol: PPID
• Gene ID (NCBI): 5481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).