PPARD Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PPARD Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10E1

Cat no : 60193-1-PBS

Synonyms

FAAR, NR1C2, NUC1, NUCI, NUCII, Nuclear hormone receptor 1, PPAR beta, PPAR delta, PPARB, PPARD

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Validation Data Gallery

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  WB analysis of A549 using 60193-1-Ig (same clone as 60193-1-PBS)

  WB result of PPARD antibody (60193-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPARD transfected A549 cells. This data was developed using the same antibody clone with 60193-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60193-1-Ig (same clone as 60193-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60193-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60193-1-Ig (same clone as 60193-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60193-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 60193-1-Ig (same clone as 60193-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60193-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SW480 using 60193-1-Ig (same clone as 60193-1-PBS)

  SW480 cells were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60193-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig adipose using 60193-1-Ig (same clone as 60193-1-PBS)

  pig adipose tissue were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60193-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60193-1-PBS targets PPARD in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPARD fusion protein Ag16770
• Full Name: peroxisome proliferator-activated receptor delta
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC002715
• Gene Symbol: PPARD
• Gene ID (NCBI): 5467
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.