PPARD Monoclonal antibody

• Featured Product
• KD/KO Validated

PPARD Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, ChIP, ELISA

Conjugate

Unconjugated

Publications

9

CloneNo.

1B10E1

Cat no : 60193-1-Ig

Synonyms

FAAR, NR1C2, NUC1, NUCI, NUCII, Nuclear hormone receptor 1, PPAR beta, PPAR delta, PPARB, PPARD

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Validation Data Gallery

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  WB analysis using 60193-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 60193-1-Ig

  WB result of PPARD antibody (60193-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPARD transfected A549 cells.

• 

  WB analysis using 60193-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 60193-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SW480 using 60193-1-Ig

  SW480 cells were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig adipose using 60193-1-Ig

  pig adipose tissue were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, pig adipose tissue, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• ChIP: See 1 publications below

Product Information

60193-1-Ig targets PPARD in WB, ChIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPARD fusion protein Ag16770
• Full Name: peroxisome proliferator-activated receptor delta
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC002715
• Gene Symbol: PPARD
• Gene ID (NCBI): 5467
• RRID: AB_10896827
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.

Publications

Species	Application	Title
mouse	WB	Nat Commun; A proteomics landscape of circadian clock in mouse liver.; Authors - Yunzhi Wang
mouse	WB	Liver Int; PPARβ/δ activation protects against hepatic ischaemia-reperfusion injury; Authors - Baolin Qian ; KD Validated
mouse	WB	Am J Physiol Endocrinol Metab; Zinc finger protein 407 overexpression upregulates PPAR target gene expression and improves glucose homeostasis in mice; Authors - Alyssa Charrier
mouse	WB	Front Aging Neurosci; A tau fragment links depressive-like behaviors and cognitive declines in Alzheimer's disease mouse models through attenuating mitochondrial function; Authors - Yamei Wang
rat	WB	J Ethnopharmacol; Anchang Yuyang Decoction inhibits experimental colitis-related carcinogenesis by regulating PPAR signaling pathway and affecting metabolic homeostasis of host and microbiota; Authors - Xiunan Wei
human	WB,ChIP	Adv Sci (Weinh); The Loss of an Orphan Nuclear Receptor NR2E3 Augments Wnt/β-catenin Signaling via Epigenetic Dysregulation that Enhances Sp1-β catenin-p300 Interactions in Hepatocellular Carcinoma; Authors - Yuet-Kin Leung