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  • Featured Product
  • KD/KO Validated

PPARA Monoclonal antibody, PBS Only

PPARA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G1E10

Cat no : 66826-1-PBS

Synonyms

PPAR alpha, PPAR, Peroxisome proliferator-activated receptor alpha, NR1C1, hPPAR

Validation Data Gallery

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  • Western Blot (WB) analysis of ROS1728 cells using PPARA Monoclonal antibody (66826-1-Ig)

    WB analysis of ROS1728 using 66826-1-Ig (same clone as 66826-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66826-1-Ig (PPARA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66826-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using PPARA Monoclonal antibody (66826-1-Ig)

    WB analysis of HSC-T6 using 66826-1-Ig (same clone as 66826-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66826-1-Ig (PPARA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66826-1-PBS in a different storage buffer formulation.

Product Information

66826-1-PBS targets PPARA in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPARA fusion protein Ag7896
Full Name peroxisome proliferator-activated receptor alpha
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC000052
Gene Symbol PPARA
Gene ID (NCBI) 5465
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Peroxisome proliferator-activated receptor alpha (PPARA) is a ligand-activated transcription factor that belongs to the PPAR nuclear receptor superfamily. PPARA is essential in the modulation of lipid transport and metabolism, mainly through activating mitochondrial and peroxisomal fatty acid β-oxidation pathways. In addition, PPARA seems to decrease inflammation mainly through direct interaction with NF-κB, causing inhibition of its signaling pathway or reducing the activated levels of NF-κB and subsequent inflammation. Furthermore, PPARA was implicated in the attenuation of oxidative stress in alcoholic liver disease when treated with polyenephosphatidylcholine through downregulation of ROS-generating enzymes such as ethanol-inducible cytochrome P450 2E1 (CYP2E1), acyl-CoA oxidase, and NADPH oxidase. PPARA exists two isoforms and molecular weight of PPARA isoforms are 52 kDa and 22 kDa. The ability of a retinoid X receptor (RXR) to heterodimerize with many nuclear receptors, including LXR, PPAR, NGF1B and RAR, underscores its pivotal role within the nuclear receptor superfamily. Among these heterodimers, PPAR:RXR is considered an important signalling mediator of both PPAR ligands, such as fatty acids, and 9-cis retinoic acid (9-cis RA), an RXR ligand. (PMID: 15103326 ). PPARA can form Heterodimer with RXRA and molecular weight of Heterodimer is about 110 kDa.