PPARA Monoclonal antibody

• Featured Product
• KD/KO Validated

PPARA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (6)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

73

CloneNo.

1G1E10

Reviews (1)

Cat no : 66826-1-Ig

Synonyms

hPPAR, NR1C1, PPAR, PPAR alpha, PPARA, PPARα

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Validation Data Gallery

• 

  WB analysis of ROS1728 using 66826-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66826-1-Ig (PPARA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66826-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66826-1-Ig (PPARA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HSC-T6 cells, ROS1728 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 69 publications below
• IHC: See 9 publications below
• IF: See 1 publications below

Product Information

66826-1-Ig targets PPARA in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish, goat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPARA fusion protein Ag7896
• Full Name: peroxisome proliferator-activated receptor alpha
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC000052
• Gene Symbol: PPARA
• Gene ID (NCBI): 5465
• RRID: AB_2882169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxisome proliferator-activated receptor alpha (PPARA) is a ligand-activated transcription factor that belongs to the PPAR nuclear receptor superfamily. PPARA is essential in the modulation of lipid transport and metabolism, mainly through activating mitochondrial and peroxisomal fatty acid β-oxidation pathways. In addition, PPARA seems to decrease inflammation mainly through direct interaction with NF-κB, causing inhibition of its signaling pathway or reducing the activated levels of NF-κB and subsequent inflammation. Furthermore, PPARA was implicated in the attenuation of oxidative stress in alcoholic liver disease when treated with polyenephosphatidylcholine through downregulation of ROS-generating enzymes such as ethanol-inducible cytochrome P450 2E1 (CYP2E1), acyl-CoA oxidase, and NADPH oxidase. PPARA exists two isoforms and molecular weight of PPARA isoforms are 52 kDa and 22 kDa. The ability of a retinoid X receptor (RXR) to heterodimerize with many nuclear receptors, including LXR, PPAR, NGF1B and RAR, underscores its pivotal role within the nuclear receptor superfamily. Among these heterodimers, PPAR:RXR is considered an important signalling mediator of both PPAR ligands, such as fatty acids, and 9-cis retinoic acid (9-cis RA), an RXR ligand. (PMID: 15103326 ). PPARA can form Heterodimer with RXRA and molecular weight of Heterodimer is about 110 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease; Authors - Evangelia Lekka
human,mouse	WB	Research (Wash D C); Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis; Authors - Lang Linghu ; KD Validated
human	WB,IHC	Cell Death Differ; RUNX2 recruits the NuRD(MTA1)/CRL4B complex to promote breast cancer progression and bone metastasis.; Authors - Xin Yin
rat	WB	Phytomedicine; Podophyllotoxin via SIRT1/PPAR /NF-κB axis induced cardiac injury in rats based on the toxicological evidence chain (TEC) concept; Authors - Tao Jiang
human	IHC	Front Pharmacol; A novel risk model of three SUMOylation genes based on RNA expression for potential prognosis and treatment sensitivity prediction in kidney cancer; Authors - Song-Chao Li
mouse	WB	J Cell Physiol; Dynein light chain LC8 alleviates nonalcoholic steatohepatitis by inhibiting NF-κB signaling and reducing oxidative stress.; Authors - Gong-Rak Lee

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tianyi (Verified Customer) (08-30-2024)

Antigen retrieval in Tris/EDTA buffer and secondary antibody in AF647.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Ovarian tissue

PPARA Antibody Immunofluorescence validation (1:100 dilution) in Ovarian tissue (Cat no:66826-1-Ig)