PPAR Gamma Monoclonal antibody, PBS Only

PPAR Gamma Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F4A2

Cat no : 66936-1-PBS

Synonyms

CIMT1, NR1C3, PPAR gamma, PPARG, PPARG1, PPARG2, PPARgamma

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Validation Data Gallery

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  WB analysis using 66936-1-Ig (same clone as 66936-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66936-1-Ig (PPARG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66936-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66936-1-Ig (same clone as 66936-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66936-1-Ig (PPAR Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66936-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse adipose using 66936-1-Ig (same clone as 66936-1-PBS)

  mouse adipose tissue were subjected to SDS PAGE followed by western blot with 66936-1-Ig (PPAR Gamma antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66936-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66936-1-Ig (same clone as 66936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66936-1-Ig (PPAR Gamma antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66936-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66936-1-Ig (same clone as 66936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66936-1-Ig (PPAR Gamma antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66936-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66936-1-Ig (same clone as 66936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66936-1-Ig (PPAR Gamma antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66936-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66936-1-Ig (same clone as 66936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66936-1-Ig (PPAR Gamma antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66936-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66936-1-Ig (same clone as 66936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66936-1-Ig (PPAR Gamma antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66936-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66936-1-Ig (same clone as 66936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66936-1-Ig (PPAR Gamma antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66936-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66936-1-PBS targets PPAR Gamma in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPAR Gamma fusion protein Ag16657
• Full Name: peroxisome proliferator-activated receptor gamma
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC006811
• Gene Symbol: PPARG
• Gene ID (NCBI): 5468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, INS resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 INS-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus (PMID: 15689403). Experimental data indicate that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa) (PMID: 10922459). PPARG has been reported to be localized mainly (but not always) in the nucleus. PPARG can also be detected in the cytoplasm and was reported to possess extra-nuclear/non-genomic actions (PMID: 17611413; 19432669; 14681322).