POU4F3 Recombinant antibody, PBS Only

POU4F3 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230345F1

Cat no : 82980-1-PBS

Synonyms

BRN3C, Brn 3C, Brain-specific homeobox/POU domain protein 3C, Brain-3C, Brain 3C

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Validation Data Gallery

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  WB analysis using 82980-1-RR (same clone as 82980-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82980-1-RR (POU4F3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82980-1-RR (same clone as 82980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82980-1-RR (POU4F3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82980-1-RR (same clone as 82980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82980-1-RR (POU4F3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 82980-1-RR (same clone as 82980-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using POU4F3 antibody (82980-1-RR, Clone: 230345F1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 82980-1-RR (same clone as 82980-1-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human POU4F3 (82980-1-RR, Clone:230345F1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82980-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82980-1-RR against Human POU4F3 were performed. The affinity constant is 0.234 nM.

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Product Information

82980-1-PBS targets POU4F3 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: POU4F3 fusion protein Ag16004
• Full Name: POU class 4 homeobox 3
• Calculated Molecular Weight: 338 aa, 37 kDa
• Observed Molecular Weight: 33-40 kDa
• GenBank Accession Number: BC104923
• Gene Symbol: POU4F3
• Gene ID (NCBI): 5459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.