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POU4F3 Recombinant antibody

POU4F3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230345F1

Cat no : 82980-1-RR

Synonyms

BRN3C, Brn 3C, Brain-specific homeobox/POU domain protein 3C, Brain-3C, Brain 3C

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using human POU4F3 Recombinant antibody (82980-1-RR)

    WB analysis using 82980-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82980-1-RR (POU4F3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using POU4F3 Recombinant antibody (82980-1-RR)

    IHC staining of mouse brain using 82980-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82980-1-RR (POU4F3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using POU4F3 Recombinant antibody (82980-1-RR)

    IHC staining of mouse brain using 82980-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82980-1-RR (POU4F3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using POU4F3 Recombinant antibody (82980-1-RR)

    IF Staining of U2OS using 82980-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using POU4F3 antibody (82980-1-RR, Clone: 230345F1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of U-251 cells using POU4F3 Recombinant antibody (82980-1-RR)

    FC experiment of U-251 using 82980-1-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human POU4F3 (82980-1-RR, Clone:230345F1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 82980-1-RR

    Affinity and Kinetic Characterization of 82980-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82980-1-RR against Human POU4F3 were performed. The affinity constant is 0.234 nM.

Tested Applications

Positive WB detected inNeuro-2a cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82980-1-RR targets POU4F3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen POU4F3 fusion protein Ag16004
Full Name POU class 4 homeobox 3
Calculated Molecular Weight 338 aa, 37 kDa
Observed Molecular Weight 33-40 kDa
GenBank Accession NumberBC104923
Gene Symbol POU4F3
Gene ID (NCBI) 5459
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POU4F3 antibody 82980-1-RRDownload protocol
IHC protocol for POU4F3 antibody 82980-1-RRDownload protocol
IF protocol for POU4F3 antibody 82980-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols