POT1 Recombinant antibody

POT1 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240688E5

Cat no : 83732-2-RR

Synonyms

POT1-like telomere end-binding protein, hPot1, 240688E5

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Validation Data Gallery

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  IF Staining of HeLa using 83732-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POT1 antibody (83732-2-RR, Clone: 240688E5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 83732-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POT1 antibody (83732-2-RR, Clone: 240688E5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of U2OS using 83732-2-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug POT1 Recombinant antibody (83732-2-RR, Clone:240688E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of SH-SY5Y using 83732-2-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug POT1 Recombinant antibody (83732-2-RR, Clone:240688E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83732-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83732-2-RR against Human POT1 were performed. The affinity constant is 2.57 nM.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: U2OS cells, SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83732-2-RR is POT1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: POT1 fusion protein Ag0875
• Full Name: POT1 protection of telomeres 1 homolog (S. pombe)
• Calculated Molecular Weight: 71 kDa
• GenBank Accession Number: BC002923
• Gene Symbol: POT1
• Gene ID (NCBI): 25913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protection of telomeres 1(POT1) is a conserved protein that binds the G-rich stand of its own telomeric repeat sequence, with a role in protecting chromosome ends. It's housekeeping gene that is required to ensure the integrity of chromosome ends in all cells. It's a component of the telomerase ribonucleoprotein(RNP) complex that is esential for the replication of chromosome termini. It consists the TRF1 compex that is involved in the regulation of telomere length by cis-inhibition of telomerase. We have discovered that POT1 exists in at least three consistently occurring forms; 90, 70 and 45kDa. The unexpected molecular weights of POT1 seem to be associated with SUMO1 and ubiquitin conjugation; the latter occurring at a double lysine residue at 289-KK-290 (.PMID: 24054699)