POT1 Polyclonal antibody, PBS Only

POT1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10581-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 10581-1-AP (same clone as 10581-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10581-1-AP (POT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10581-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 10581-1-AP (same clone as 10581-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10581-1-AP (POT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10581-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10581-1-AP (same clone as 10581-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10581-1-AP (POT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10581-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10581-1-AP (same clone as 10581-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10581-1-AP (POT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10581-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10581-1-AP (same clone as 10581-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10581-1-AP (POT1 antibody) at dilution of 1:100 (under 25x lens). This data was developed using the same antibody clone with 10581-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 10581-1-AP (same clone as 10581-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10581-1-AP (POT1 Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10581-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 10581-1-AP (same clone as 10581-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10581-1-AP (POT1 Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10581-1-PBS in a different storage buffer formulation.

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Product Information

10581-1-PBS targets POT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POT1 fusion protein Ag0875
• Full Name: POT1 protection of telomeres 1 homolog (S. pombe)
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 45 kDa, 71 kDa
• GenBank Accession Number: BC002923
• Gene Symbol: POT1
• Gene ID (NCBI): 25913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Protection of telomeres 1(POT1) is a conserved protein that binds the G-rich stand of its own telomeric repeat sequence, with a role in protecting chromosome ends. It's housekeeping gene that is required to ensure the integrity of chromosome ends in all cells. It's a component of the telomerase ribonucleoprotein(RNP) complex that is esential for the replication of chromosome termini. It consists the TRF1 compex that is involved in the regulation of telomere length by cis-inhibition of telomerase. We have discovered that POT1 exists in at least three consistently occurring forms; 90, 70 and 45kDa. The unexpected molecular weights of POT1 seem to be associated with SUMO1 and ubiquitin conjugation; the latter occurring at a double lysine residue at 289-KK-290 (.PMID: 24054699)