POT1 Polyclonal antibody

POT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 10581-1-AP

Synonyms

hPot1, POT1

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Validation Data Gallery

• 

  WB analysis of HeLa using 10581-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10581-1-AP (POT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10581-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10581-1-AP (POT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10581-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10581-1-AP (POT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10581-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10581-1-AP (POT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 10581-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10581-1-AP (POT1 antibody) at dilution of 1:100 (under 25x lens).

• 

  IHC staining of human cervical cancer using 10581-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10581-1-AP (POT1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10581-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10581-1-AP (POT1 Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 4 publications below
• IP: See 1 publications below

Product Information

10581-1-AP targets POT1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POT1 fusion protein Ag0875
• Full Name: POT1 protection of telomeres 1 homolog (S. pombe)
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC002923
• Gene Symbol: POT1
• Gene ID (NCBI): 25913
• RRID: AB_2284057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protection of telomeres 1(POT1) is a conserved protein that binds the G-rich stand of its own telomeric repeat sequence, with a role in protecting chromosome ends. It's housekeeping gene that is required to ensure the integrity of chromosome ends in all cells. It's a component of the telomerase ribonucleoprotein(RNP) complex that is esential for the replication of chromosome termini. It consists the TRF1 compex that is involved in the regulation of telomere length by cis-inhibition of telomerase. We have discovered that POT1 exists in at least three consistently occurring forms; 90, 70 and 45kDa. The unexpected molecular weights of POT1 seem to be associated with SUMO1 and ubiquitin conjugation; the latter occurring at a double lysine residue at 289-KK-290 (.PMID: 24054699)

Publications

Species	Application	Title
human	IHC	Aging (Albany NY); SIRT6 enhances telomerase activity to protect against DNA damage and senescence in hypertrophic ligamentum flavum cells from lumbar spinal stenosis patients.; Authors - Jianwei Chen
human	IP	Cell Cycle; CGGBP1 phosphorylation constitutes a telomere-protection signal.; Authors - Umashankar Singh
human	WB	Sci Rep; Concomitant underexpression of TGFBR2 and overexpression of hTERT are associated with poor prognosis in cervical cancer.; Authors - Hui Yang
human	WB	Front Cell Dev Biol; Telomere Attrition in Induced Pluripotent Stem Cell-Derived Neurons From ALS/FTD-Related C9ORF72 Repeat Expansion Carriers.; Authors - Hayley Robinson
human	WB	J Cancer; Downregulation of Ubiquitin-conjugating Enzyme UBE2D3 Promotes Telomere Maintenance and Radioresistance of Eca-109 Human Esophageal Carcinoma Cells.; Authors - Hui Yang
mouse	IHC	Exp Gerontol; The altered expression of telomerase components and telomere-linked proteins may associate with ovarian aging in mouse.; Authors - Esra Gozde Kosebent