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PON1 Recombinant antibody, PBS Only (Capture)

PON1 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241091B7

Cat no : 83982-2-PBS

Synonyms

paraoxonase 1, K-45, EC:3.1.1.2, Aromatic esterase 1, A-esterase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PON1 Recombinant antibody (83982-2-RR)

    WB analysis using 83982-2-RR (same clone as 83982-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83982-2-RR (PON1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83982-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00877-2

    Cytometric bead array standard curve of MP00877-2

    Cytometric bead array standard curve of MP00877-2, PON1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83982-2-PBS. Detection antibody: 83982-1-PBS. Standard: Ag12934. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83982-2-RR

    Affinity and Kinetic Characterization of 83982-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83982-2-RR against Human PON1 were performed. The affinity constant is below 1 pM.

Product Information

83982-2-PBS targets PON1 as part of a matched antibody pair:

MP00877-2: 83982-2-PBS capture and 83982-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PON1 fusion protein Ag12934
Full Name paraoxonase 1
Calculated Molecular Weight 355 aa, 40 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberBC074719
Gene Symbol PON1
Gene ID (NCBI) 5444
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PON1, also named PON and K-45, belongs to the paraoxonase family. PON1 hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is capable of hydrolyzing a broad spectrum of organophosphate substrates and several aromatic carboxylic acid esters. PON1 may mediate enzymatic protection of low-density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. PON1 was identified as a triple band in a range of approximately 35-40 kDa. For glycosylated, the MW of PON1 is migrated 42-45kd, while the 35 kDa band represents the unglycosylated form (PMID: 17906223, 32002976, 21852972). The antibody has cross-reaction to PON2 and PON3.