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PON1 Recombinant antibody

PON1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241091B7

Cat no : 83982-2-RR

Synonyms

paraoxonase 1, K-45, EC:3.1.1.2, Aromatic esterase 1, A-esterase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PON1 Recombinant antibody (83982-2-RR)

    WB analysis using 83982-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83982-2-RR (PON1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83982-2-RR

    Affinity and Kinetic Characterization of 83982-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83982-2-RR against Human PON1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inhuman plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83982-2-RR is PON1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PON1 fusion protein Ag12934
Full Name paraoxonase 1
Calculated Molecular Weight 355 aa, 40 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberBC074719
Gene Symbol PON1
Gene ID (NCBI) 5444
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PON1, also named PON and K-45, belongs to the paraoxonase family. PON1 hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is capable of hydrolyzing a broad spectrum of organophosphate substrates and several aromatic carboxylic acid esters. PON1 may mediate enzymatic protection of low-density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. PON1 was identified as a triple band in a range of approximately 35-40 kDa. For glycosylated, the MW of PON1 is migrated 42-45kd, while the 35 kDa band represents the unglycosylated form (PMID: 17906223, 32002976, 21852972). The antibody has cross-reaction to PON2 and PON3.

Protocols

Product Specific Protocols
WB protocol for PON1 antibody 83982-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols