ACTH Recombinant monoclonal antibody

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  IHC staining of mouse pituitary gland using 82498-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue slide using 82498-2-RR (POMC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse pituitary gland using 82498-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pituitary gland tissue using POMC antibody (82498-2-RR, Clone: 250672H3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACTH Uni-rAb^® Recombinant Antibody for IHC, IF-P, ELISA

Cat No. 82498-2-RR

Clone No.250672H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Synonyms

POMC, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma, Beta-endorphin

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Tested Applications

• Positive IHC detected in: mouse pituitary gland tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse pituitary gland tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82498-2-RR targets ACTH in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: proopiomelanocortin
• Calculated Molecular Weight: 267 aa, 29 kDa
• GenBank Accession Number: BC065832
• Gene Symbol: POMC
• Gene ID (NCBI): 5443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01189
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.