ACTH Recombinant monoclonal antibody, PBS Only

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  IHC staining of mouse pituitary gland using 82498-2-RR (same clone as 82498-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue slide using 82498-2-RR (POMC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82498-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse pituitary gland using 82498-2-RR (same clone as 82498-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pituitary gland tissue using POMC antibody (82498-2-RR, Clone: 250672H3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82498-2-PBS in a different storage buffer formulation.

ACTH Uni-rAb^® Recombinant Antibody for IHC, IF-P, Indirect ELISA

Cat No. 82498-2-PBS

Clone No.250672H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, Indirect ELISA

Synonyms

POMC, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma, Beta-endorphin

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Product Information

82498-2-PBS targets ACTH in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: proopiomelanocortin
• Calculated Molecular Weight: 267 aa, 29 kDa
• GenBank Accession Number: BC065832
• Gene Symbol: POMC
• Gene ID (NCBI): 5443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01189
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.