POLD2 Polyclonal antibody

POLD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29765-1-AP

Synonyms

DNA polymerase delta subunit 2

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Validation Data Gallery

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  WB analysis using 29765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 29765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 29765-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 29765-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 29765-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 29765-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 29765-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLD2 antibody (29765-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293T cells, NIH/3T3 cells, HeLa cells, Jurkat cells, SGC-7901 cells, U-251 cells, mouse colon tissue, mouse pancreas tissue, rat colon tissue
• Positive IHC detected in: human colon tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29765-1-AP targets POLD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLD2 fusion protein Ag31521
• Full Name: polymerase (DNA directed), delta 2, regulatory subunit 50kDa
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 48-51 kDa
• GenBank Accession Number: BC000459
• Gene Symbol: POLD2
• Gene ID (NCBI): 5425
• RRID: AB_2918344
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLD2, also named as DNA polymerase delta subunit 2, is a 469 amino acid protein, which belongs to the DNA polymerase delta/II small subunit family. The function of the small subunit is not yet clear. POLD2 has an essential function for the small subunit in the heterodimeric core enzyme.