POGZ Polyclonal antibody, PBS Only

POGZ Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30106-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 30106-1-AP (same clone as 30106-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30106-1-AP (POGZ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 30106-1-AP (same clone as 30106-1-PBS)

  IP result of anti-POGZ (IP:30106-1-AP, 4ug; Detection:30106-1-AP 1:2000) with HeLa cells lysate 1800 ug. This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 30106-1-AP (same clone as 30106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 30106-1-AP (same clone as 30106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 30106-1-AP (same clone as 30106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 30106-1-AP (same clone as 30106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 30106-1-AP (same clone as 30106-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POGZ antibody (30106-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 30106-1-AP (same clone as 30106-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using POGZ antibody (30106-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

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Product Information

30106-1-PBS targets POGZ in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POGZ fusion protein Ag32435
• Full Name: pogo transposable element with ZNF domain
• Calculated Molecular Weight: 155kd
• Observed Molecular Weight: 155 kDa
• GenBank Accession Number: NM_015100
• Gene Symbol: POGZ
• Gene ID (NCBI): 23126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

POGZ, also known as MRD37, is a zinc-finger protein containing 9 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. POGZ plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. POGZ plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms (PMID:20562864). Heterozygous mutation of POGZ causes White-Sutton syndrome (PMID: 26739615). Expect a band approximately 155 kDa in size corresponding to POGZ by western blotting.