POGZ Polyclonal antibody

POGZ Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 30106-1-AP

Synonyms

KIAA0461, POGZ, SUHW5, ZNF280E, ZNF635, ZNF635m

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Validation Data Gallery

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  WB analysis using 30106-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30106-1-AP (POGZ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 30106-1-AP

  IP result of anti-POGZ (IP:30106-1-AP, 4ug; Detection:30106-1-AP 1:2000) with HeLa cells lysate 1800 ug.

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  IHC staining of mouse brain using 30106-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 30106-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 30106-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 30106-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 30106-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POGZ antibody (30106-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of U-251 using 30106-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using POGZ antibody (30106-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, SH-SY5Y cells, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30106-1-AP targets POGZ in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POGZ fusion protein Ag32435
• Full Name: pogo transposable element with ZNF domain
• Calculated Molecular Weight: 155kd
• Observed Molecular Weight: 155 kDa
• GenBank Accession Number: NM_015100
• Gene Symbol: POGZ
• Gene ID (NCBI): 23126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POGZ, also known as MRD37, is a zinc-finger protein containing 9 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. POGZ plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. POGZ plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms (PMID:20562864). Heterozygous mutation of POGZ causes White-Sutton syndrome (PMID: 26739615). Expect a band approximately 155 kDa in size corresponding to POGZ by western blotting.