CoraLite®594-conjugated POFUT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

POFUT1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-14929

Synonyms

O FucT 1, O Fuc T, KIAA0180, GDP-fucose protein O-fucosyltransferase 1, FUT12

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Validation Data Gallery

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  IF Staining of HUVEC using CL594-14929

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite®594 POFUT1 antibody (CL594-14929) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-14929

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 POFUT1 antibody (CL594-14929) at dilution of 1:200.

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  FC experiment of A431 using CL594-14929

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human POFUT1 (CL594-14929) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HUVEC cells, HepG2 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-14929 targets POFUT1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POFUT1 fusion protein Ag6710
• Full Name: protein O-fucosyltransferase 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC000582
• Gene Symbol: POFUT1
• Gene ID (NCBI): 23509
• RRID: AB_2919815
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

POFUT1 (protein O-fucosyltransferase 1), also known as GDP-fucose protein O-fucosyltransferase, is an enzyme that catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains and plays a crucial role in Notch signaling.