CoraLite®594-conjugated PNPLA3 Monoclonal antibody

PNPLA3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D2B11

Cat no : CL594-67369

Synonyms

Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, iPLA(2)epsilon, iPLA2 epsilon, PNPLA3

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Validation Data Gallery

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  IF Staining of HeLa using CL594-67369

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PNPLA3 antibody (CL594-67369, Clone: 1D2B11 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67369 targets PNPLA3 in IF/ICC applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PNPLA3 fusion protein Ag27407
• Full Name: patatin-like phospholipase domain containing 3
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC065195
• Gene Symbol: PNPLA3
• Gene ID (NCBI): 80339
• RRID: AB_2920121
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

PNPLA3 belongs to a family of proteins that share a domain that was first identified in patatin, a major soluble protein of potato tubers with nonspecific acyl hydrolase activity. The domain differs from classical lipases by employing a catalytic dyad (Ser-Asp), rather than a catalytic triad, to effect hydrolysis. PNPLA3 is expressed primarily in liver and adipose tissue, in which it partitions to membranes and lipid droplets. Real-time PCR of cDNA from human tissues indicated that PNPLA3 expression was highest in the liver, followed by skin and adipose tissue. PNPLA3 is expressed at the highest levels in adipose tissue of mice. The PNPLA3 protein was expected at 50-70 kDa, as observed; the additional band at approximately 130-150 kDa is a oligomer bands. (PMID: 20385813, PMID: 24931521, PMID: 23023705)