PNMT Recombinant antibody

PNMT Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241111G1

Cat no : 83979-5-RR

Synonyms

PNMTase, Phenylethanolamine N-methyltransferase, PENT, Noradrenaline N-methyltransferase, EC:2.1.1.28

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Validation Data Gallery

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  WB analysis of K-562 using 83979-5-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 83979-5-RR (PNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83979-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83979-5-RR against Human PNMT were performed. The affinity constant is 8.83 pM.

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Tested Applications

• Positive WB detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83979-5-RR targets PNMT in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PNMT fusion protein Ag4098
• Full Name: phenylethanolamine N-methyltransferase
• Calculated Molecular Weight: 282 aa, 31 kDa
• GenBank Accession Number: BC037246
• Gene Symbol: PNMT
• Gene ID (NCBI): 5409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PNMT (Phenylethanolamine N-methyltransferase) is the final enzyme in the catecholamine synthesizing cascade that converts noradrenaline to epinephrine by transferring a methyl group from S-adenosyl-L-methionine (SAM) to norepinephrine. PNMT is primarily found in the adrenal medulla, where it is responsible for the production of epinephrine. It is also present in certain areas of the brain, such as the amygdala and retina, where it may be involved in various neurophysiological functions (PMID: 17175506). Variations in the PNMT gene have been associated with Alzheimer's disease, hypertension, and other disorders (PMID: 2816488,.11514309).