PMS2 Monoclonal antibody

PMS2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

12

CloneNo.

1G4E6

Reviews (1)

Cat no : 66075-1-Ig

Synonyms

HNPCC4, PMS1 protein homolog 2, PMS2, PMS2CL, PMSL2

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Validation Data Gallery

• 

  WB analysis of HeLa using 66075-1-Ig

  Vairous cells were subjected to SDS PAGE followed by western blot with 66075-1-Ig (PMS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66075-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66075-1-Ig (PMS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66075-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66075-1-Ig (PMS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 9 publications below

Product Information

66075-1-Ig targets PMS2 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PMS2 fusion protein Ag12661
• Full Name: PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
• Calculated Molecular Weight: 862 aa, 96 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC093921
• Gene Symbol: PMS2
• Gene ID (NCBI): 5395
• RRID: AB_11182595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS).

Publications

Species	Application	Title
mouse	IHC	Cancer Immunol Res; Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite Stability.; Authors - Qijing Wu
human	IHC	Front Oncol; Molecular Classification in Patients With Endometrial Cancer After Fertility-Preserving Treatment: Application of ProMisE Classifier and Combination of Prognostic Evidence.; Authors - Xuting Ran
human	WB,IHC	Mol Carcinog; The mechanism of RGS5 regulating gastric cancer mismatch repair protein; Authors - Zhenwei Yang
human	WB	Sci Rep; Mismatch repair proteins play a role in ATR activation upon temozolomide treatment in MGMT-methylated glioblastoma.; Authors - Sachita Ganesa
human	IHC	Surgery; Mismatch repair status as a beneficial predictor of fluorouracil-based adjuvant chemotherapy for pancreatic cancer.; Authors - Dingkong Liang
human	IHC	J Surg Oncol; Clinicopathological and survival characteristic of mismatch repair status in ovarian clear cell carcinoma.; Authors - Jun Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniela (Verified Customer) (03-17-2022)

Bands in WB and IF staining were both aspecific.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for WB; 1:100 for IF
• Cell Tissue Type: Gastric mouse organoids and oesophageal cancer cell line

PMS2 Antibody Western Blot,Immunofluorescence validation (1:1000 for WB; 1:100 for IF dilution) in Gastric mouse organoids and oesophageal cancer cell line (Cat no:66075-1-Ig)