PMS1 Recombinant antibody, PBS Only

PMS1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240408D9

Cat no : 83456-6-PBS

Synonyms

PMSL1, MLH2, hPMS1, HNPCC3, 240408D9

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Validation Data Gallery

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  WB analysis using 83456-6-RR (same clone as 83456-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83456-6-RR (PMS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83456-6-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83456-6-RR (same clone as 83456-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83456-6-RR (PMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83456-6-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83456-6-RR (same clone as 83456-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83456-6-RR (PMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83456-6-PBS in a different storage buffer formulation.

• 

  IF Staining of Caco-2 using 83456-6-RR (same clone as 83456-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PMS1 antibody (83456-6-RR, Clone: 240408D9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83456-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83456-6-RR (same clone as 83456-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PMS1 antibody (83456-6-RR, Clone: 240408D9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83456-6-PBS in a different storage buffer formulation.

• 

  IF Staining of Caco-2 using 83456-6-RR (same clone as 83456-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PMS1 antibody (83456-6-RR, Clone: 240408D9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83456-6-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83456-6-RR (same clone as 83456-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PMS1 Recombinant antibody (83456-6-RR, Clone:240408D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83456-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83456-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83456-6-RR against Human PMS1 were performed. The affinity constant is 0.367 nM.

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Product Information

The immunogen of 83456-6-PBS is PMS1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PMS1 fusion protein Ag28132
• Full Name: PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
• Observed Molecular Weight: 106 kDa
• GenBank Accession Number: BC096331
• Gene Symbol: PMS1
• Gene ID (NCBI): 5378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PMS1 Homolog 1 is a member of the DNA mismatch repair enzymes (MMREs) which is to eliminate the mismatch of insertions and deletions as a consequence of DNA polymerase errors at DNA synthesis in eukaryotes. PMS1 and MLH1 could form a heterodimer MutLβ that belongs to MMREs (PMID:25619773). In the MMR system, the MutS complex recognizes the DNA mispairs firstly, and then the Mlh1-Pms1 and other associated proteins such as PCNA, RFC, ExoI were recruited, inducing Exonuclease 1 (Exo1)-dependent and -independent MMR pathways (PMID:24981171). Mutations in this gene cause hereditary nonpolyposis colorectal cancer(HNPCC), which is also known as Lynch syndrome. It was reported that PMS1 is a widely expressed protein and located in the nucleus. Alternative splicing allows for 4 isoforms of PMS1 protein.