PLXDC2 Recombinant antibody

PLXDC2 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240174A11

Cat no : 83449-4-RR

Synonyms

UNQ2514/PRO6003, Tumor endothelial marker 7-related protein, TEM7R, Plexin domain-containing protein 2, plexin domain containing 2

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Validation Data Gallery

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  WB analysis of MKN-45 using 83449-4-RR

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 83449-4-RR (PLXDC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83449-4-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83449-4-RR (PLXDC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 83449-4-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83449-4-RR (PLXDC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83449-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83449-4-RR against Human PLXDC2 were performed. The affinity constant is 42.5 pM.

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Tested Applications

• Positive WB detected in: MKN-45 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83449-4-RR is PLXDC2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PLXDC2 fusion protein Ag2940
• Full Name: plexin domain containing 2
• Calculated Molecular Weight: 480 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC012885
• Gene Symbol: PLXDC2
• Gene ID (NCBI): 84898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLXDC2 (Plexin-domain containing 2), also named tumor endothelial marker 7-related protein (TEM7R), is a single-pass type I membrane protein with some homology to nidogen and to plexins. PLXDC2 is located in tumor endothelium and plays a role in tumor angiogenesis. Three isoforms of PLXDC2 produced by alternative splicing have been described. Recent finding revealed that PLXDC2 is a mitogen for neural progenitors and functions as a novel regulator of the development of different brain regions, suggesting its role in coordinating proliferation and differentiation in the developing nervous system.