PLS1 Polyclonal antibody, PBS Only

PLS1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13143-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 13143-1-AP (same clone as 13143-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13143-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13143-1-PBS in a different storage buffer formulation.

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  IHC staining of Human Colon cancer using 13143-1-AP (same clone as 13143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 13143-1-AP (PLS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13143-1-PBS in a different storage buffer formulation.

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  IHC staining of Human Colon cancer using 13143-1-AP (same clone as 13143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 13143-1-AP (PLS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13143-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 13143-1-AP (same clone as 13143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13143-1-AP (PLS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13143-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 13143-1-AP (same clone as 13143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13143-1-AP (PLS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13143-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 13143-1-AP (same clone as 13143-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLS1 antibody (13143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13143-1-PBS in a different storage buffer formulation.

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Product Information

13143-1-PBS targets PLS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLS1 fusion protein Ag3532
• Full Name: plastin 1 (I isoform)
• Calculated Molecular Weight: 629 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC031083
• Gene Symbol: PLS1
• Gene ID (NCBI): 5357
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PLS1 also known as Fimbrin, Plastin 1, is an actin-binding protein in the absence of calcium. In the inner ear, PLS1 is required for stereocilia formation. PLS1 Mediates liquid packing of actin filaments that is necessary for stereocilia to grow to their proper dimensions. PLS1 is specifically expressed at high levels in the small intestine, colon, and kidney; relatively expressed at lower levels in the lung and stomach(PMID: 8139549). PLS1 is required for normal hearing in humans and mice. Mutations in PLS1, encoding fimbrin, cause autosomal dominant nonsyndromic hearing loss(NSHL)(PMID: 31397523).