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PLS1 Polyclonal antibody

PLS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13143-1-AP

Synonyms

Plastin-1, Plastin 1, I-plastin, Intestine-specific plastin, I plastin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLS1 Polyclonal antibody (13143-1-AP)

    WB analysis using 13143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13143-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of Human Colon cancer tissue using PLS1 Polyclonal antibody (13143-1-AP)

    IHC staining of Human Colon cancer using 13143-1-AP

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 13143-1-AP (PLS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human Colon cancer tissue using PLS1 Polyclonal antibody (13143-1-AP)

    IHC staining of Human Colon cancer using 13143-1-AP

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 13143-1-AP (PLS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLS1 Polyclonal antibody (13143-1-AP)

    IHC staining of human kidney using 13143-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13143-1-AP (PLS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLS1 Polyclonal antibody (13143-1-AP)

    IHC staining of human kidney using 13143-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13143-1-AP (PLS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PLS1 Polyclonal antibody (13143-1-AP)

    IF Staining of A549 using 13143-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLS1 antibody (13143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, mouse colon tissue, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, Human Colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13143-1-AP targets PLS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLS1 fusion protein Ag3532
Full Name plastin 1 (I isoform)
Calculated Molecular Weight 629 aa, 70 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC031083
Gene Symbol PLS1
Gene ID (NCBI) 5357
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLS1 also known as Fimbrin, Plastin 1, is an actin-binding protein in the absence of calcium. In the inner ear, PLS1 is required for stereocilia formation. PLS1 Mediates liquid packing of actin filaments that is necessary for stereocilia to grow to their proper dimensions. PLS1 is specifically expressed at high levels in the small intestine, colon, and kidney; relatively expressed at lower levels in the lung and stomach(PMID: 8139549). PLS1 is required for normal hearing in humans and mice. Mutations in PLS1, encoding fimbrin, cause autosomal dominant nonsyndromic hearing loss(NSHL)(PMID: 31397523).

Protocols

Product Specific Protocols
WB protocol for PLS1 antibody 13143-1-APDownload protocol
IHC protocol for PLS1 antibody 13143-1-APDownload protocol
IF protocol for PLS1 antibody 13143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols